To determine statistically significant differential expression between treatment conditions, we used “Ballgown” (Pertea et al. 2016), an R Bioconductor package. No genes or transcripts were removed from analyses and the adjusted P-value (a.k.a. q-value) was set to the standard false discovery rate < 0.05. All pairwise condition contrasts were examined at both the gene and transcript level. Only two genes were found to be differentially expressed 24 hr after the various treatments, the actin filament nucleating protein cappuccino (capu) (air vs. odors) (Figure 1D) and the nuclear DNA-binding protein pre-mod(mdg4)-G (odors vs. EtOH) (Figure 1E).
