To uncover experience-dependent effects on transcription, we examined genes that showed the greatest variance in expression (log-transformed counts per million means) across repeated air, ethanol, odors, and paired ethanol-odor (trained) treatments. Among the 100 most variable genes, many ribosomal proteins and previously uncharacterized (unnamed CG#) genes were identified (Figure S3D). Also included were Esterase-6 (Est-6), Cytochrome P450-4g1 (Cyp4g1), la costa (lcs), Mucin related 18B (Mur18B), alpha and beta Trypsin (αTry, βTry), yippee interacting protein 7 (yip7), fab1 kinase (fab1), Troponin C (TpnC73F and TpnC47D), Odorant binding protein 51a (Obp51a), obstructor-B (obst-B), inaF-B (inaF-B), Autophagy-related 10 (Atg-10), short spindle 2 (ssp2), neurotransmitter transporter-like (Ntl), Multiple inositol polyphosphate phosphatase 2 (Mipp2), and a number of Jonah peptidase transcripts (Figure 1C). Excluding RNA-associated genes further revealed Maltase Ai (Mal-A1), Sarcolamban B (SclB), Cuticular protein 49Ab (Cpr49Ab), and Coiled-coil domain containing 56 (Ccdc56) (Figure 1C).
