Having these orthogonally generated mRNA quantification data we sought to first cross-validate these data with our differentially expressed genes observed from the PsychGene NanoString molecular profiles of the CXCR4+ NPCs. As summarized in Supplementary Figure 14, of the 15 genes analyzable by both methods, we observed concordant results for four (80%) of the five upregulated transcripts and six (60%) of the downregulated transcripts. Given the vastly different nature of the assays, with discrepancies being possible due to differences in transcripts considers and sensitivity of the methods, these results overall provide confidence in the reproducibility of the molecular profiles.
