Next, to determine whether the set of 503 transcripts covered any significantly overrepresented Gene Ontology (GO) terms suggestive of common process and pathways, we analyzed the set using DAVIDv6.7 bioinformatics software (Table 1; Sup. Table 11). Of the 412 transcripts that could be mapped, 18 GO terms associated with the category of Biological Process were found to be significantly overrepresented (>3×; p<0.05, Benjamini-Hochberg) with the most enriched being those concerning Neuron Differentiation, Development, and Morphogenesis. At the same level of enrichment and significance, a total of six Cellular Compartment terms were overrepresented, including multiple terms covering the extracellular matrix and chromatin. Taken together, these data are overall supportive of our conclusions from the molecular profiling using the PsychGene NanoString probe set that are differences in expression of genes important for neurodevelopment and neurogenesis in the BD patient CXCR4+ NPCs compared to their unaffected parental controls.
