Chunk #88 — STAR★Methods — Quantification and Statistical Analysis — Cytograph pipeline

Source

Molecular Architecture of the Mouse Nervous System.

Embedded

yes

Text

Chromium samples were sequenced, typically one sample per lane, per the manufacturer’s instructions with one 98 bp read located near the 3ʹ end of the mRNA. Illumina runs were demultiplexed, aligned to the genome and mRNA molecules were counted using the 10X Genomics cellranger pipeline.