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Chunk #53 — ONLINE METHODS — DNA looping using Hi-C

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Genome-wide association analyses identify 44 risk variants and refine the genetic architecture of major depression.
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Dorsolateral prefrontal cortex (Brodmann area 9) was dissected from postmortem samples from three adults of European ancestry (Dr Craig Stockmeier, University of Mississippi Medical Center). Cerebra from three fetal brains were obtained from the NIH NeuroBiobank (URLs; gestation age 17–19 weeks, African ancestry). We used “easy Hi-C” to assess DNA chromatin (looping) interactions (see Supplementary Note).