Chunk #37 — MATERIALS AND METHODS — Preparation of explants and transplantation

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Musculocontractural Ehlers-Danlos syndrome and neurocristopathies: dermatan sulfate is required for Xenopus neural crest cells to migrate and adhere to fibronectin.

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Explants enriched in neural crest and epidermis were prepared by removing the chorion membrane with forceps, hemi-sectioning stage 18 embryos, and cutting off the neural epithelium and yolk with a surgical blade (Swann-Morton 10, REF 0301) on 1% agarose plates in MBS.