CNC explants from the embryos injected into the animal hemisphere with eGFP mRNA at stage 17 were dissected and homotypically grafted into host embryos as previously described (Borchers et al., 2000) with the following modifications: the dissection and grafting of the CNC explants were performed on 1% agarose in MBS. At ∼30 min after grafting, the embryo was transferred to a clean dish filled with 0.1× MBS and incubated at 17°C. At stage 26, the migration pattern of the transplanted CNC cells was monitored with a fluorescence stereomicroscope (Leica MZFLIII) and documented with a digital camera (Nikon DS-Fi1c).
