Chunk #14 — Materials and methods — Screening for functional SNPs in the 3′UTR

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Allele-specific expression and high-throughput reporter assay reveal functional genetic variants associated with alcohol use disorders.

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To identify the functional 3′UTR SNPs, i.e. those whose ratio between reference and alternative allele was significantly different between the vector-expressed RNA and the plasmid DNA extracted from the same transfected cells, a generalized linear model was used. Similar to ASE analysis, a negative binomial distribution was used to account for the over-dispersion effects on the UMI count data.