from w1118 null flies (Figure S2A), indicating strong knockdown of mini-w. Ethanol sensitivity in ethanol sedation assays was statistically indistinguishable in w1118 nulls, flies expressing mini-w (elav-Gal4/+ and v5597/+ controls) and w knockdown flies (elav-Gal4,v30034/+) (Figure 5A). Expression of Cnx14D RNAi v5597 with elav-Gal4 (elav-Gal4/v5597) led to the expected increase in ST50, but importantly this phenotype was not significantly affected by coincident knockdown of mini-w (elav-Gal4,v30034/v5597) (Figure 5A). Similarly, we found that ethanol sensitivity in w1118, elav-Gal4,v30034/+, and elav-Gal4/+ controls were comparable and that the increased sensitivity of flies expressing ph-p RNAi v50024 was not affected by concurrent knockdown of mini-w (Figure 5B). Although additional studies will be required to determine if knock-down of Cnx14D and ph-p alter ethanol sensitivity in flies, these data show that mini-w has no significant effect on the phenotypes of flies with increased or decreased sensitivities to ethanol.
