We next examined the effects of endogenous w on sensitivity and rapid tolerance to ethanol in ethanol sedation assays. We found no differences in initial ethanol sensitivity in w1118 null and w wild-type (w+) females or males tested with vapor from 50% (Figure 6A and B) or 40% ethanol (Figure S8A). The development of rapid tolerance to ethanol was similarly unaffected by w genotype in either sex (Figure S8B and C). Additionally, there were no significant differences in internal ethanol concentrations (Figure 6C and 6D) in w1118 and w+ flies. These results indicate that endogenous w, like mini-w, has no discernible effect on ethanol sensitivity, rapid ethanol tolerance or ethanol kinetics in ethanol sedation assays.
