Chunk #45 — ONLINE METHODS — Post-mortem samples — “Penn” sample - University of Pennsylvania Brain Bank of Psychiatric illnesses and Alzheimer’s Disease Core Center (http://www.med.upenn.edu/cndr/biosamples-brainbank.shtml)
Brain specimens are obtained from the Penn prospective collection. Disease diagnoses were made based on DSM-IV criteria and obtained through a clinical interview by psychiatrist and review of medical records. All procedures for Penn are approved by the Committee on Studies Involving Human Beings of the University of Pennsylvania, and the use of control postmortem tissues was considered exempted research in accordance with CFR 46.101 (b), item 65 of Federal regulations and University policy. At autopsy, the right or left hemisphere of each brain is blocked into coronal slabs, which are immediately frozen and stored at −80°C. For this study, Brodmann areas 9/46 were dissected from either the left or right hemisphere and pulverized in liquid nitrogen. The tissue was shipped in tubes appropriate for DNA or RNA extraction to ISMMS as homogenized tissue in trizol for RNA extraction and as dry pulverized tissue for DNA extraction.
