Alcohol self-administration data and Ki-67, DCX, AC-3, BrdU and FJC data comparing nondependent alcohol self-administering and alcohol dependent groups were analyzed by non-matching or one-way analysis of variance (ANOVA), phenotypic analysis were analyzed by two-way ANOVA using GraphPad Prism 5. All analyses were followed by Bonferroni's or Tukey's post hoc tests. Values of p < 0.05 were considered statistically significant.