Chunk #18 — MATERIALS AND METHODS — PSD length measurements

Source

Endogenous GluR1-containing AMPA receptors translocate to asymmetric synapses in the lateral amygdala during the early phase of fear memory formation: an electron microscopic immunocytochemical study.

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In order to determine whether the changes in GluR1 or GluR2/3 immunoreactivity could result from the increased rate of detection due to the enlargement of PSD lengths, randomly encountered PSDs from each animal were subjected to PSD length measurements. The segmented line tool of ImageJ (1.37J, NIH) was used to measure PSD lengths. All images were digital, captured at a magnification of 50,000×, by using the same source of tissue that underwent GluR1- and GluR2/3-immunore-activity analysis.