Chunk #52 — STAR Methods — Experimental Model and Subject Details — Cell Culture — Differentiation of iHPCs to iMGLs

Source

iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases.

Embedded

yes

Text

After 25 days of microglial differentiation (35 days from iPSC), iMGLs were cultured in complete differentiation media supplemented with CD200 (100 ng/ml, Novoprotein) and CX3CL1 (100 ng/ml; Peprotech) for an additional three days before use in studies.