All studies using human tissue were approved by the McGill University institutional review board (McGill University Health Centre Ethics Board; #ANTJ2001/1). All experiments were conducted in accordance with the Helsinki Declaration. Sex of donor was either not provided or collected. Human microglia were isolated from adult brain tissue using previously described protocols (Durafourt et al., 2013). Briefly, normal appearing cortical tissue was resected from pharmacologically intractable non-malignant cases of temporal lobe epilepsy. Tissue was cleaned extensively and mechanically dissociated. A single cell suspension was generated following gentle enzymatic digestion using trypsin and DNAse prior to passing through a nylon mesh filter. Single cell suspension underwent a fickle ultracentrifugation step to remove myelin. Dissociated cells were centrifuged, counted, and plated at 2×106 cells/mL in MEM supplemented with heat-inactivated FBS (5%), P/S (0.1% v/v) and glutamine (0.1% v/v.). Microglia were grown for 3 days, collected and plated at 1×105 cells/mL and maintained in culture for 6 days during which time cells received two treatments of TGFβ (20 ng/mL) on days 3 and 5. Human fetal brain tissue was obtained from the Fetal
