To measure each participant’s response to alcohol, the Subjective High Assessment Scale (SHAS) questionnaire was administered. For the challenge, each subject was given 8 minutes to consume a 20% by volume solution of 95% ethanol, at 0.75 ml/kg for women and 0.9 ml/kg for men. Baseline levels for each score (SHAS, body sway, and breath alcohol level) were measured prior to the challenge and then were measured at multiple set time intervals throughout the 3 hour challenge. Ultimately the changes in SHAS score and body sway at 1 hour after the challenge were used as phenotypes for the genome-wide genetic analysis. Genotyping was performed on 811 microsatellite markers across the genome.
