Genomic DNA was extracted from whole peripheral blood samples. Genotyping was performed on 10 SNPs with Taqman genotyping assays with locus specific PCR primers and fluorescent allele specific probes designed by Applied Biosystems. Standard Taqman protocol was followed and endpoint amplification intensity was measured by the 7900 ABI Sequence Detector. The position of the genotyped markers in relation to CYP2E1 can be found in Figure 1. The HapMap Consortium reported three major haplotypes in the Caucasian population, as seen in Figure 1, which could be distinguished by the initial two SNPs that were genotyped. Table 4 lists the names and positions of the genotyped SNPs.
