When generating iPSCs from blood cells, it has to be distinguished between the use of peripheral blood and umbilical cord blood. In case of using adult peripheral blood, there are two options for harvesting the starting material for reprogramming. One way is to use mobilized CD34+ peripheral blood cells isolated in a process similar to the procedure which is routinely performed in stem cell donation [15]. In the preparation phase, the donor has to inject himself the granulocyte colony-stimulating factor (G-CSF), a growth factor which is commonly present in the human body, on five consecutively following days [53]. The process before the actual isolation of the starting material is time-consuming, cost intensive, and painful for the cell donor. In the human body, G-CSF is strongly involved in the regulation of the hematopoietic system, particularly stimulating the production of white blood cells. The overdose of this cytokine leads to an increase in the production and release (or mobilization) of hematopoietic stem cells from the bone marrow into the peripheral blood. In a process called aphaeresis, the CD34 positive stem cells can
