Chunk #13 — Development of the protocol: Quality control of recovered clones

Source: Efficient, footprint-free human iPSC genome editing by consolidation of Cas9/CRISPR and piggyBac technologies.
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We also investigated the potential for piggyBac excision to leave residual genomic scars. Our analysis (Suppl. Data) confirmed scarless piggyBac excision in all clones that we examined.