Sodium bisulfite mapping was performed as previously described[55]. After gene-specific PCR amplification (Table S2) of sodium bisulfite treated DNA for each subject, a mix of 10 ng of the gel-extracted PCR product from all of the subjects from each High and Low LG group (n = 3/group) were used for subsequent molecular cloning (Cequation 8800, Beckman-Coulter). We obtained 20 clones for sequencing from 2–3 independent PCR reactions for each subject.
