The genes verified for differences in DNA methylation by qCHIP analysis were further analyzed for genotyping using identical primers (Table S2). The resulting PCR products for each subject were sequenced bidirectionally using the forward and the reverse primer by Genome Quebec (ABI 3100, Applied Biosystems). Genetic variation was assessed throughout the PCR amplicon used for qCHIP analysis by alignment of genomic DNA with the published gene sequence (CLC Workbench, CLC bio).
