P and r-values of numerically significant correlations are shown in the Table S3. DNA-binding activity of constitutively active NF-κB positively correlated with IKKβ protein content in the PFC of both alcoholics and control subjects (Figure 4A). The p65 and p50 protein levels positively correlated in the PFC of the control group while the correlation was weaker in alcoholics (Figure 4B). Correlations between the six mRNAs analyzed were evaluated in each cortex and for alcoholics and control subjects, respectively (Table S3). Four correlations in the PFC of both alcoholics and control subjects had r-values that exceeded 0.8 and P<0.0001; RELA vs. NFKB1 (Figure 4C), RELA vs. IKKβ, NFKB1 vs. IKKβ and RELA vs. IκBα (Table S3). The interrelationship of the six NF-κB mRNA levels and levels of transcripts of the tumor necrosis factor receptor 1A, p53, myelin basic protein and DNA fragmentation factor α genes in the PFC were evaluated for comparison; no or poor correlations were found with most of them having r-values ranging from 0.1 to 0.6.
