OxoSQ infused into the LDTg mildly increased locomotor activity, when measured as mean total distance traveled in cm. Although repeated measures ANOVA identified a significant main effect of dose (Fig 5A) for the 60-min time bin [F (2, 14) = 4.34, p = 0.034], Bonferroni corrected pairwise mean comparisons for the 60-min bin identified only a statistical trend (p = 0.071) for a difference in activity between OxoSQ 10 nmol and aCSF treated rats (Fig 5A). That any effects on activity were small in magnitude was supported by the lack of a statistically significant interaction of time bin and treatment, when all five time bins were included in the analysis (Fig 5A). The total distance traveled during each of the time bins between baseline sessions and aCSF session did not significantly differ (Fig 5A inset). Similarly, the total distance traveled during any of the time bins between aCSF and microinjection in CIC with OxoSQ 10 nmol was not statistically different (Fig 5B).
