DCX-IR also varied as a function of time (Fig. 2; main effect: F4,40=8.5, p<0.0001). Similar to its effect on proliferation, chronic alcohol exposure decreased DCX-IR cells by the end of the exposure (day 0, p<0.001). There was a statistically non-significant trend (p=0.09) towards an increase on day 3. DCX-IR returned to control level after 7 and 21 d of abstinence.