In the dentate gyrus, the number of BrdU-IR cells varied as a function of time from exposure (Fig. 1; main effect: F4,38=11.8, p<0.0001). Post-hoc analysis showed that numbers of BrdU-IR cells were decreased at the end of alcohol exposure (day 0, p<0.01). This was followed by a burst of proliferation on day 3 (p<0.001). Proliferating cells returned to control levels on days 7 and 21 of abstinence (Fig. 1a, b). The correlation of BrdU-IR and Ki67-IR was high (R=0.61, p<0.0001), and Ki67-IR followed a temporal pattern that closely paralleled that of BrdU-IR (Fig. 1c ; main effect of time: F4,39=25.3, p<0.0001).
