To evaluate a potential underlying mechanism for the association of rs115455482 genotype with Ca-HL, the regulatory relationship between rs115455482 and CHRM3 expression was evaluated in the Braineac database. In expression quantitative trait locus (eQTL) analysis, CHRM3 was variably expressed among ten brain tissues and differentially expressed among rs115455482*T risk allele carriers in the THAL (P = 4.5 × 10−4; adjusted P = 4.5 × 10−3 across ten brain tissues) and PUTM (P = 1.6 × 10−2; adjusted P = 1.6 × 10−1 across ten brain tissues) (Fig. 2). Rs115455482 risk genotype TC (TT homozygotes were too rare to be observed in this sample) was correlated with lower mRNA expression of CHRM3 in THAL, with the same trend found in other tissues, including PUTM, OCTX, MEDU, SNIG, FCTX, HIPP, and CRBL.
