To infer the potential biological function of CHRM3, we carried out co-expression analysis. We obtained the top 100 co-expressed genes (Supplementary Table S2) with respect to CHRM3 by application of COXPRESdb18 and then performed enrichment analysis in disease-associated gene categories in WebGestalt19, which showed that 24 genes were significantly enriched within the top 10 disease-associated gene categories (all raw P-values < 1 × 10−2 and adjusted P-values < 1 × 10−2), including psychotic disorders, epilepsy, and schizophrenia (Supplementary Information “CHRM3 Co-Expression Analysis” and Supplementary Table S3). According to GWAS database GRASP21, CHRM3 is an epilepsy risk gene and CHRM3 SNP rs10925980 was nominally associated with genetic generalized epilepsies (P = 1.90 × 10−6) and Ca-HL (P = 0.03) in EAs (see Supplementary Table S4). In addition, 24 CHRM3 co-expressed genes were significantly (all P-values < 1 × 10−2) correlated with CHRM3 in at least 1 of 10 brain tissues, with the same being true for 14 Chrm3 co-expressed genes expressed in GABAergic neurons (Supplementary Fig. 4).
