Exposure of SON neurons to alcohol (20 mM, at or just above legal intoxication levels, up to 24h) in HNS organotypic explants changed the BK variant landscape dramatically. End-point PCR, subcloning and sequencing revealed the presence of two bands (Figure 1B) encoding two variants (Figure 1D): 1) the STREX variant (STREX-1 in Xie and McCobb, 1998), and 2) a completely spliced-out variant (INSERTLESS).
