Chunk #5 — 2. Materials and Methods — 2.3. Quantitative reverse transcription PCR (qRT-PCR) analyses

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Cross-generational THC exposure alters the developmental sensitivity of ventral and dorsal striatal gene expression in male and female offspring.

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Striatal brain regions (dorsal and ventral striatum) were dissected from frozen adolescent and adult brains of rats with a 15-gauge sample punch on a cold plate based on landmarks according to commonly used atlases (Paxinos and Watson, 2007). RNA was prepared from bilateral tissue punches using the RNAqueous-Micro Kit (Thermo Fisher Scientific, Waltham, MA) and cDNA was obtained with a first-strand synthesis kit (Quanta Biosciences, Gaithersburg, MD). Quantitative real-time PCR was performed using the LightCycler480 Probes Master reagent (Roche, Basel, Switzerland) and the TaqMan PCR program in a LightCycler 480 instrument (Roche).