Chunk #6 — 2. Materials and Methods — 2.3. Quantitative reverse transcription PCR (qRT-PCR) analyses

Source

Cross-generational THC exposure alters the developmental sensitivity of ventral and dorsal striatal gene expression in male and female offspring.

Embedded

yes

Text

The following Taqman-based assays (Applied Biosystems, Foster City, CA) were used in triplicate PCR reactions: Cnr1, Rn00562880_m1; Grin1, Rn01436038_m1; Grin2A, Rn00561341_m1; Grin2B, Rn00680474_m1; Gria1, Rn00709588_m1; Gria2, Rn00568514_m1; Dlg4, Rn00571479_m1; Dlgap3, Rn00597609_m1; beta-2-microglobulin, Rn00560865_m1. Each gene of interest was run in duplex with a reference gene (beta-2-microglobulin), data were normalized via the ΔΔCT method (Livak and Schmittgen, 2001).