of samples with different values for the peak family size, we have determined that a peak family size of six maximizes the efficiency of DS: that is, requiring the smallest number of raw reads to produce a single DCS (Fig. 5a). Although a peak family size of six is optimal, it should be noted that data yield continues to increase, albeit at a decreasing overall efficiency, for peak family sizes ranging between six and 16 members (Fig. 5b). As the peak family size approaches 16 reads per tag family, nearly all additional reads are redundant and consume sequencing capacity without yielding further DCS data. Therefore, when deciding on DNA input amounts for PCR, we aim for a peak family size ranging between six and 12 members. This range allows for some tolerance in peak family size because of pipetting or measurement error, while maximizing the final number of DCSs that we obtain.
