To achieve greater sequencing depth for a given target, or to achieve the same depth for a larger genome or genome target, more DCSs are needed. To increase the number of reads obtained in DS, it is necessary to proportionally increase both the input amounts of DNA for PCR and the dedicated lane fraction to maintain a peak family size of six (Table 2). Nonproportional changes to PCR input and lane fraction lead to suboptimal peak family sizes and substantial decreases in DS efficiency (Figs. 4b,c and 5a). The choice of lane fraction (i.e., the number of reads devoted to a particular sample) will be influenced by the size of the targeted region and the desired depth of coverage. In particular, for a given lane fraction, the final sequencing depth of a sample will decrease as the size of the genome target increases. The following formula can be used to estimate the number of reads to devote to a sample: N=40DGR Where N is the number of paired-end reads devoted to a sample (i.e., lane fraction), D is the desired
