We analyzed baseline levels of CRH, ACTH and Cortisol using ANOVA. Analyses were performed with rearing condition as a co-independent variable, but since there were no interactions with genotype and because the inclusion of this variable did not reduce the residual variance, it was removed from the analyses. Alcohol consumption data were also analyzed by ANOVA. To control for potential differences in drinking patterns among test cohorts, we also generated z-scores controlling for cohort. Prior reports from this lab have shown that alcohol consumption is higher among peer-reared macaques and that functional genetic variants can interact with early rearing history to influence levels of ethanol self-administration 17, 36. Because of these established differences and the fact that males generally consume more ethanol than females, both sex and rearing condition were included as a co-independent variables in the analysis.
