With the normal legal driving limit for blood alcohol concentration being 0.08 g/L a dose of 50 mM alcohol would equate to ~ 3× the legal limit for operating a vehicle in the United States (Lieberman, Kranzler, Levine, & Covault, 2017). However, studies have also been conducted using alcohol concentrations that are lower (~ 10 mM) and higher (~ 70 mM). A 10 mM alcohol concentration is used to model aspects of social drinking behaviors (Rewal et al., 2012; Sundstrom-Poromaa et al., 2002; J. Wang et al., 2016; Wei, Faria, & Mody, 2004), while a concentration of 70 mM would correlate more to the blood alcohol concentration of an extremely heavy drinker in human (Olson, Smith, Kloss, Ho, & Apple, 2013). Concentrations above 100 mM have been found to directly cause cytotoxic effects on cells (Baker & Kramer, 1999; L. N. Li, Margolis, & Hoffman, 1991). The concentration used is critical when setting up your experimental design and would ultimately depend on the type of question(s) being asked. Finally, in addition to concentration, time dependent administration also needs to be considered. Most studies assay acute (24 hour) versus chronic (~ 7 days) alcohol exposure (Lowery-Gionta, Marcinkiewcz, & Kash, 2015; Mandrekar,
