depend on the type of question(s) being asked. Finally, in addition to concentration, time dependent administration also needs to be considered. Most studies assay acute (24 hour) versus chronic (~ 7 days) alcohol exposure (Lowery-Gionta, Marcinkiewcz, & Kash, 2015; Mandrekar, Bala, Catalano, Kodys, & Szabo, 2009). An acute exposure to alcohol can be used to mimic binge drinking, while a more chronic exposure recapitulates mild, reoccurring alcohol use. Taken together, it is important to consider these factors when designing experiments to answer specific (focused) types of questions regarding AUDs. In addition, are the toxic effects of alcohol direct or indirect?
