We studied by qRT-PCR three other genes, ANK3, CACNA1C and ODZ4 that have been implicated by genetic studies of BPD [44–46]. As with the microarray study, the mRNA for these genes did not show significant differences between BPD and controls in L neurons by qRT-PCR. Similar to our findings at 4 weeks of neural differentiation, Madison et. al. [22] did not detect differences in expression of these genes at 6 weeks using the PsychGene NanoString probe set. Bavamian et al [52] did show decreases in ANK3 gene expression in BPD lines compared to controls at 2 and 8 weeks of neural differentiation but not at 4 or 6 weeks.
