When tested 1h after the last learning trial, alcohol-exposed 3xTg-AD mice exhibited a significant memory deficit as shown by less time spent in the quadrant previously containing the escape platform (Fig. 5A, right), which is consistent with an alcohol-induced deficit in hippocampal-dependent spatial memory. Two-way ANOVA (genotype alcohol exposure) found no main effect of genotype or alcohol but identified a significant interaction between those factors [F(1, 13) = 5.018, P = 0.0432], indicating that the effects of alcohol depend on genotype. Multiple comparison procedures showed that alcohol-exposed 3xTg-AD mice spent significantly less time in the quadrant that previously contained the escape platform (Fig. 5A, right).
