CD83eGFP reporter animals were generated and kept in-house24. CD83fl/fl mice were generated as described previously16 and were bred to Cx3cr1CreErt2/+(Jung) mice for microglia-specific depletion of the Cd83 gene (CD83ΔMG). Cx3cr1-CreERT2 mice were purchased from Jackson Laboratories. CD83wt mice, which were bred with Cx3cr1-CreERT2 mice, served as control animals in all experiments (hereafter termed as CD83wtCre). For in vitro assessment of CD83-deficient microglia, we used conditional knockout mice generated with the Cx3cr1-Cre line and the appropriate Cre-mice as controls. We used an equal proportion of age-matched male and female mice in all experiments. Mice were kept under specific pathogen-free conditions at 22 °C and 40–50% humidity on a 12/12-h light/dark cycle and were fed with standard laboratory chow ad libitum. Animal care and all experimental procedures of the present study were performed in accordance with the European Community Standards on the Care and Use of Laboratory Animals and were approved by the local ethics committee (Administration of Lower Franconia; Reference number 55.2.2-2532-2-1193).
