To induce a microglia-specific deletion of the Cd83 gene, CD83ΔMG and control mice received a subcutaneous injection of 4 mg of tamoxifen (Sigma #T5648), which was solved in warm corn oil (Sigma #C8267) at a concentration of 20 mg/ml, and this procedure was repeated 48 h later. To ensure the reconstitution of CD83 expression in peripheral CX3CR1+ cells, all experiments were conducted at least 7 weeks after the last tamoxifen treatment.
