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Chunk #9 — Materials and Methods — Human subjects and sigmoid mucosal samples

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Alcohol stimulates activation of Snail, epidermal growth factor receptor signaling, and biomarkers of epithelial-mesenchymal transition in colon and breast cancer cells.
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Subjects recruited were excluded if they reported gastrointestinal diseases (except for hiatal hernia or hemorrhoids), clinically detectable ascites or severe edema, evidence of ongoing infection, major depression or anxiety requiring therapy, clinically significant lung or heart disease, or regular use of NSAIDs. All subjects had normal liver function tests and had no clinical evidence of liver disease.