Chunk #10 — Materials and Methods — Human subjects and sigmoid mucosal samples

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Alcohol stimulates activation of Snail, epidermal growth factor receptor signaling, and biomarkers of epithelial-mesenchymal transition in colon and breast cancer cells.

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Sigmoid biopsy samples were taken during an unprepped sigmoidoscopy and mucosal samples were snap frozen in liquid nitrogen, transferred to the laboratory and stored in −80°C freezer. The procedure was performed after a Rush IRB approved informed, written consent.