provided amplification buffer was added and allowed to incubate on a shaker at room temperature, protected from light for 15min. Finally, this solution was decanted, and the beads were suspended in assay buffer before being read using the Luminex® 200 system equipped with xPONENT® 3.1 software. Median fluorescence intensity was normalized against EGF stimulated control lysate and then relative quantification was calculated against the loading control.
