Alcohol solutions (25%, w/v) were prepared by diluting 95% EtOH (Pharmco Products Inc., Brookfield, CT) with a saccharin (0.1%, w/v) solution. Saccharin (0.1%, w/v; Millipore Sigma, St. Louis, MO) was prepared in water. Pentobarbital (60mg/mL) was dissolved in ddH2O and administered via intraperitoneal injection with a 27G needle.
