Chunk #52 — Methods — In vivo point mutation assay

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Alcohol and endogenous aldehydes damage chromosomes and mutate stem cells.

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The λ select-cII (BigBlue) mutagenesis assay was performed following the manufacturer’s instructions. This assay allows the detection of point mutations in vivo and is based on the ability of coliphage λ to multiply either through the lytic or lysogeneic cycles in Escherichia coli.