In brief, the RecoverEase DNA-isolation kit (Stratagene) was used to extract genomic DNA from the bone marrow of 8-to-12-week old Aldh2−/−Fancd2−/− and control mice that carry Big Blue λLIZ repeats. The shuttle vector that was recovered from mouse genomic DNA was packaged into phage with the Transpack packaging extract (Stratagene), which was then used to infect E. coli G1250 (Stratagene).
