In this study, we showed that the combination of the two transcription factors Ascl1 and Dlx2 is sufficient to convert human PSCs into essentially pure cultures of GABAergic neurons. The cells are generated in a single conversion step and reach synaptic maturity after 5 weeks. Given the importance of inhibitory neurotransmission in health and disease, AD-iN cells will likely become a critical addition in the portfolio of cell types to model diseases with human cells.
