frequency of spontaneous IPSCs in iN cells following collybistin KD (Fig. 4e–g). This effect was specific for inhibitory synapses, as there was no change in spontaneous EPSCs or intrinsic membrane properties (Supplementary Fig. 9). These findings are similar to findings in mouse collybistin mutant neurons24. Thus, the GABAergic iN cells are a valuable tool for studying inhibitory synaptic transmission in human neurons.
