Chunk #56 — Methods — Differential expression analysis — Single-cell specificity and cell type enrichment analysis

Source

Analysis of gene expression in the postmortem brain of neurotypical Black Americans reveals contributions of genetic ancestry.

Embedded

yes

Text

We next expanded our cell type enrichment analysis to single-cell datasets with glial (that is, astrocyte, microglia and oligodendrocyte) subtype annotation and non-brain immune cells (that is, peripheral blood mononuclear cells (PBMCs)). For the glial subpopulations, we downloaded human postmortem hippocampus astrocyte, microglia and oligodendrocyte lineage single-cell data25 from the UCSC cell browser76. For PBMCs, we downloaded human PBMC single-cell data24 from Zenodo (10.5281/zenodo.4273999).