Chunk #21 — Results — Associations of DNA Methylation with Physical Aggression in the Loci of Transcriptional Regulators of Differentially Expressed Cytokines
The methylation profile of the STAT6 locus in T cells is illustrated in Figure 4. Two regions in this locus are more methylated in the T cells of CPA individuals compared to controls (Figure 4A and Figure S6, highlighted in blue). The first region is located at the transcription start site of the STAT6 isoform 2 gene (Figure 4 left bottom panel), and the second region is located 1 Kb upstream of STAT6 isoform 1 (Figure 4 right bottom panel). Both regions coincide with DNase hypersensitive clusters and many transcription factor binding sites such as IRF4, HEY1 and NFkB. Enrichment for H3K4Me3 and H3K27Ac marks were found in the first region (TSS isoform 1) in both cell lines whereas only a small peak of H3K4Me1 enrichment was found in the second region in the GM12878 cell line.
